HumanKine® recombinant human PDGFbb protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.3-3 ng/mL

Specific Activity

minimally 5.00 x 10⁶ IU/mg

Species Reactivity

human,mouse,rat

Purity

>95%

Publications

11

Cat no : HZ-1308

Synonyms

Becaplermin, c sis, FLJ12858, PDGF 2, PDGF subunit B, PDGF2, PDGFB, PDGFbb, Proto oncogene c Sis, SIS, SSV

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Validation Data Gallery

• 

  Purity of recombinant human PDGFbb was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human PDGFbb (HZ-1308) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.1% FBS for 24 hours before treatment with increasing concentrations of recombinant human PDGFbb for 48 hrs. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 0.3-3 ng/mL.

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Technical Specifications

• GeneID: 5155
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.3-3 ng/mL
• Specific Activity: minimally 5.00 x 10^6 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P31240
• Molecular Mass: 14 and 36 kDa reduced, 29 to 32 kDa non-reduced, homodimer, glycosylated
• Formulation: 50mM Acetate pH 3.5 + 500mM NaCl, See Certificate of Analysis for details
• Species Reactivity: human,mouse,rat

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

PDGFbb is a member of the platelet-derived growth family factor. Four members of this family are mitogenic factors for cells of mesenchymal origin, and they are characterized by a motif of eight cysteines. PDGFbb is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, and placenta among others. PDGFbb plays a role in blood vessel development. It also implements its role in wound healing (PMID: 29266203; 28851707; 28556368).

Synonyms

Becaplermin, c sis, FLJ12858, PDGF 2, PDGF subunit B, PDGF2, PDGFB, PDGFbb, Proto oncogene c Sis, SIS, SSV

Publications

Species	Title
human	Br J Pharmacol; A novel HSF1 activator ameliorates nonalcoholic steatohepatitis by stimulating mitochondrial adaptive oxidation; Authors - Yong Rao
mouse	J Neuroinflammation; SU16f inhibits fibrotic scar formation and facilitates axon regeneration and locomotor function recovery after spinal cord injury by blocking the PDGFRβ pathway.; Authors - Ziyu Li
mouse	Front Pharmacol; Deletion of Smooth Muscle Lethal Giant Larvae 1 Promotes Neointimal Hyperplasia in Mice.; Authors - Ya Zhang
human	Arch Biochem Biophys; Quiescin/sulfhydryl oxidase 1b (QSOX1b) induces migration and proliferation of vascular smooth muscle cells by distinct redox pathways.; Authors - Karime C França
human	Commun Biol; KLF4-PFKFB3-driven glycolysis is essential for phenotypic switching of vascular smooth muscle cells; Authors - Xinhua Zhang
	FASEB J; Deficiency of thrombospondin-2 alleviates intimal hyperplasia in mice by modulating vascular smooth muscle cell proliferation and migration; Authors - Xu Li