HumanKine® recombinant human PDGFbb protein- GMP grade

• HEK293 expressed
• Endotoxin-free
• Animal-component free

EC50

0.3-3 ng/mL

Specific Activity

minimally 5.00 x 10⁶ IU/mg, typically 2.10 x 10⁷ IU/mg

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1308-GMP

Synonyms

Becaplermin, c sis, FLJ12858, PDGF 2, PDGF subunit B, PDGF2, PDGFB, PDGFbb, Proto oncogene c Sis, SIS, SSV

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Validation Data Gallery

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  Purity of recombinant human PDGFbb was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  GMP-grade recombinant human PDGFbb (HZ-1308-GMP) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.1% FBS for 24 hours before treatment with increasing concentrations of recombinant human PDGFbb for 72hrs. The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.3-3 ng/mL.

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Technical Specifications

• GeneID: 5155
• Species: Human
• Expression: HEK293
• EC50: 0.3-3 ng/mL
• Specific Activity: minimally 5.00 x 10^6 IU/mg, typically 2.10 x 10^7 IU/mg
• Purity: >95%
• Endotoxin: <0.1 EU/μg
• Accession Number: P01127
• Molecular Mass: 14 and 36 kDa reduced, 29 to 32 kDa non-reduced, homodimer, glycosylated
• Formulation: 50mM Acetate pH 3.5 + 500mM NaCl, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

PDGFbb is a member of the platelet-derived growth family factor. Four members of this family are mitogenic factors for cells of mesenchymal origin, and they are characterized by a motif of eight cysteines. PDGFbb is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, and placenta among others. PDGFbb plays a role in blood vessel development. It also implements its role in wound healing (PMID: 29266203; 28851707; 28556368).

Synonyms

Becaplermin, c sis, FLJ12858, PDGF 2, PDGF subunit B, PDGF2, PDGFB, PDGFbb, Proto oncogene c Sis, SIS, SSV