ChromoTek pSpot7 vector

The plasmid pSpot7 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot7, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.

Vector type

yeast expression vector

Promoter

TPI1

Reporter

Spot-Tag® N-terminal

Cat no : ev-7

Synonyms

pSpot7 vector, Spot vector

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Validation Data Gallery

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  TPI1 promoter: 7232-9 MCS: 14-73 Spot-Tag®: 73-108 Stop codon (TAA): 109-111 T7 primer site: 223-241 Leucin marker (LEU2): 1557-2663 CEN replication origin: 3391-3909 Ampicillin resistance gene: 4041-4901 pRB322 replication origin: 5056-5675

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  Multiple cloning site (MCS)

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Product Information

The plasmid pSpot7 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot7, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.

• Applications: Protein expression
• Vector type: yeast expression vector
• Promoter: TPI1
• Replication: 2µ (S. cerevisiae) pBR322 (E. coli)
• Reporter: Spot-Tag® (PDRVRAVSHWSS) N-terminal
• Selection: leucin (S. cerevisiae) ampicillin (E. coli)