ChromoTek pSpot5 vector

The plasmid pSpot5 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot5, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.

Vector type

yeast expression vector

Promoter

TPI1

Reporter

Spot-Tag® N-terminal

Publications

1

Cat no : ev-5

Synonyms

pSpot5 vector, Spot vector

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Validation Data Gallery

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  TPI1 promoter: 7239-9 Start codon (ATG): 10-12 Spot-Tag®: 13-48 MCS: 47-112 T7 primer site: 230-248 Leucin marker (LEU2): 1564-2670 CEN replication origin: 3398-3916 Ampicillin resistance gene: 4048-4908 pRB322 replication origin: 5063-5682

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  Multiple cloning site (MCS)

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Product Information

The plasmid pSpot5 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot5, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.

• Applications: Protein expression
• Vector type: yeast expression vector
• Promoter: TPI1
• Replication: CEN (S. cerevisiae) pBR322 (E. coli)
• Reporter: Spot-Tag® (PDRVRAVSHWSS) N-terminal
• Selection: leucin (S. cerevisiae) Ampicillin (E. coli)6

Publications

Application	Title
	Life Sci Alliance; The stabilized Pol31-Pol3 interface counteracts Pol32 ablation with differential effects on repair.; Authors - Kenji Shimada