ChromoTek pSpot2 vector

The plasmid pSpot2 is an expression vector for Spot-Tag® fusion proteins in E. coli. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot2, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the C-terminus of the POI.

Vector type

bacterial expression vector

Promoter

T7

Reporter

Spot-Tag® C-terminal

Publications

2

Cat no : ev-2

Synonyms

pSpot2 vector, Spot vector

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Validation Data Gallery

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  T7 promoter: 1-19 Lac operon: 19-46 RBS: 64-80 MCS: 90-140 Spot-Tag®: 141-176 Stop codon (TAA): 177-179 rrnB terminator: 361-518 Kanamycin resistance gene: 798-1613 pRB322 replication origin: 1781-2400

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  Multiple cloning site (MCS)

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Product Information

The plasmid pSpot2 is an expression vector for Spot-Tag® fusion proteins in E. coli. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot2, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the C-terminus of the POI.

• Applications: Protein expression
• Vector type: bacterial expression vector
• Promoter: T7
• Replication: pBR322
• Reporter: Spot-Tag® (PDRVRAVSHWSS) C-terminal
• Selection: kanamycin

Publications

Application	Title
	Cells; The Phosphoarginine Phosphatase PtpB from Staphylococcus aureus Is Involved in Bacterial Stress Adaptation during Infection.; Authors - Mohamed Ibrahem Elhawy
	Int J Mol Sci; Characterization of the Secreted Acid Phosphatase SapS Reveals a Novel Virulence Factor of Staphylococcus aureus That Contributes to Survival and Virulence in Mice; Authors - Nour Ahmad-Mansour