hIST1 Monoclonal antibody, PBS Only

hIST1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E12A7

Cat no : 66989-1-PBS

Synonyms

hIST1, IST1, IST1 homolog, KIAA0174, OLC1

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Validation Data Gallery

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  WB analysis using 66989-1-Ig (same clone as 66989-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66989-1-Ig (same clone as 66989-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 66989-1-Ig (same clone as 66989-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66989-1-Ig (same clone as 66989-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66989-1-Ig (same clone as 66989-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66989-1-Ig (same clone as 66989-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using hIST1 antibody (66989-1-Ig, Clone: 1E12A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66989-1-PBS targets hIST1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: hIST1 fusion protein Ag28364
• Full Name: KIAA0174
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC000430
• Gene Symbol: hIST1
• Gene ID (NCBI): 9798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member.