Gamma Catenin Polyclonal antibody

Gamma Catenin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27872-1-AP

Synonyms

ARVD12, Catenin gamma, CTNNG, Desmoplakin 3, Desmoplakin III, DP3, DPIII, junction plakoglobin, JUP, PDGB, PKGB, γ-Catenin

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Validation Data Gallery

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  WB analysis using 27872-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27872-1-AP (Gamma Catenin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27872-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27872-1-AP (gamma catenin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27872-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27872-1-AP (gamma catenin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 27872-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 27872-1-AP (gamma catenin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27872-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27872-1-AP (Gamma Catenin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27872-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27872-1-AP (Gamma Catenin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 27872-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Gamma Catenin antibody (27872-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 27872-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 27872-1-AP (gamma catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 27872-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Gamma Catenin antibody (27872-1-AP) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of MCF-7 using 27872-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 27872-1-AP (gamma catenin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HT-29 cells, HUVEC cells, T-47D cells, mouse heart tissue, MCF-7 cells, rat heart tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells, A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

27872-1-AP targets Gamma Catenin in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Gamma Catenin fusion protein Ag27394
• Full Name: junction plakoglobin
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC000441
• Gene Symbol: JUP
• Gene ID (NCBI): 3728
• RRID: AB_2881000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Oncol (Dordr); The POU2F1/miR-4490/USP22 axis regulates cell proliferation and metastasis in gastric cancer.; Authors - Yizhi Xiao
human	WB,IF	Int J Biol Macromol; Frameshift variants in the C-terminal of CTNNB1 cause familial exudative vitreoretinopathy by AXIN1-mediated ubiquitin-proteasome degradation condensation; Authors - Yining Liu