Dynamitin (69-376 AA) Polyclonal antibody

Dynamitin (69-376 AA) Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10030-2-AP

Synonyms

DCTN 50, DCTN2, DCTN50, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, DYNAMITIN, Dynamitin (69-376 AA), p50 dynamitin, RBP50

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 10030-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10030-2-AP (Dynamitin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10030-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10030-2-AP (Dynamitin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10030-2-AP

  IP result of anti-Dynamitin (69-376 AA) (IP:10030-2-AP, 4ug; Detection:10030-2-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human gliomas using 10030-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10030-2-AP (Dynamitin (69-376 AA) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10030-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10030-2-AP (Dynamitin (69-376 AA) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 10030-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10030-2-AP (Dynamitin (69-376 AA) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 10030-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10030-2-AP (Dynamitin (69-376 AA) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10030-2-AP targets Dynamitin (69-376 AA) in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: fusion protein
• Full Name: dynactin 2 (p50)
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC000718
• Gene Symbol: Dynamitin
• Gene ID (NCBI): 10540
• RRID: AB_513348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is against the internal region (69-376aa) of full length p50 dynamitin.