c-SRC Monoclonal antibody, PBS Only

c-SRC Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, hamster, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5E10C4

Cat no : 60315-1-PBS

Synonyms

ASV, c SRC, c-SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1

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Validation Data Gallery

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  WB analysis using 60315-1-Ig (same clone as 60315-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60315-1-Ig (c-SRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

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  WB analysis using 60315-1-Ig (same clone as 60315-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60315-1-Ig (c-SRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

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  WB analysis of CHO using 60315-1-Ig (same clone as 60315-1-PBS)

  CHO cells were subjected to SDS PAGE followed by western blot with 60315-1-Ig (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 60315-1-Ig (same clone as 60315-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60315-1-Ig (c-SRC Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig (c-SRC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig (c-SRC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig (c-SRC Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig (c-SRC Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 60315-1-Ig (same clone as 60315-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 60315-1-Ig(c-SRC antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 60315-1-Ig (same clone as 60315-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using c-SRC antibody (60315-1-Ig, Clone: 5E10C4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60315-1-PBS targets c-SRC in WB, IF, IHC, ELISA applications and shows reactivity with human, hamster, mouse, rat samples.

• Tested Reactivity: human, hamster, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: c-SRC fusion protein Ag22306
• Full Name: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC011566
• Gene Symbol: SRC
• Gene ID (NCBI): 6714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.