Filter: AllWBIHCIF/ICC

WB analysis using 60315-1-Ig (same clone as 60315-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60315-1-Ig (c-SRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.
WB analysis using 60315-1-Ig (same clone as 60315-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60315-1-Ig (c-SRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.
WB analysis of RAW 264.7 using 60315-1-Ig (same clone as 60315-1-PBS)
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60315-1-Ig (c-SRC Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.
WB analysis of CHO using 60315-1-Ig (same clone as 60315-1-PBS)
CHO cells were subjected to SDS PAGE followed by western blot with 60315-1-Ig (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig (c-SRC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig (c-SRC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig (c-SRC Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig (c-SRC Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 60315-1-Ig (same clone as 60315-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using c-SRC antibody (60315-1-Ig, Clone: 5E10C4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

c-SRC Monoclonal antibody, PBS Only

c-SRC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 60315-1-PBS

Clone No.5E10C4

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, hamster

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SRC, pp60c-src, p60-Src, EC:2.7.10.2, cSRC

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60315-1-PBS targets c-SRC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

Tested Reactivity	human, mouse, rat, hamster
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	c-SRC fusion protein Ag22306 Predict reactive species
Full Name	v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Calculated Molecular Weight	60 kDa
Observed Molecular Weight	60 kDa
GenBank Accession Number	BC011566
Gene Symbol	SRC
Gene ID (NCBI)	6714
ENSEMBL Gene ID	ENSG00000197122
RRID	AB_2881426
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P12931
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.