c-SRC Polyclonal antibody

c-SRC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, hamster, human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 25978-1-AP

Synonyms

ASV, c SRC, c-SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1

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Validation Data Gallery

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  WB analysis of A431 using 25978-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25978-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of CHO using 25978-1-AP

  CHO cells were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25978-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 25978-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25978-1-AP (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 25978-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25978-1-AP (c-SRC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25978-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25978-1-AP (c-SRC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, CHO cells, COLO 320 cells, Jurkat cells, mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

25978-1-AP targets c-SRC in WB, IHC, ELISA applications and shows reactivity with mouse, hamster, human samples.

• Tested Reactivity: mouse, hamster, human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: c-SRC fusion protein Ag22415
• Full Name: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC011566
• Gene Symbol: SRC
• Gene ID (NCBI): 6714
• RRID: AB_2880316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.

Publications

Species	Application	Title
human	WB	Life Sci; Design and screening of a novel neuropilin-1 targeted penetrating peptide for anti-angiogenic therapy in glioma.; Authors - Lin Zhao
human	WB,IHC	J Transl Med; Evodiamine inhibits proliferation and induces apoptosis of nasopharyngeal carcinoma cells via the SRC/ERBB2-mediated MAPK/ERK signaling pathway; Authors - Jie Liu
human	WB	Oncotarget; Targeting claudin-4 enhances CDDP-chemosensitivity in gastric cancer.; Authors - Yukiko Nishiguchi