ChromoTek anti-IL6 recombinant VHH, for 1x Cys conjugation

IL6Cys1 is an unconjugated recombinant anti IL6 Nanobody (VHH). Suitable for for dual cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteines reduced.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

Conjugation

Conjugate

Unconjugated

Cat no : IL6Cys1

Synonyms

IL-6 VHH, IL-6 single domain antibody (sdAb), Interleukin 6, BSF-2, CDF

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Validation Data Gallery

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  BLI analysis of the interaction between varying concentrations of anti-IL-6 VHH and immobilised, biotinylated HumanKine IL-6 (HZ-1019). Fit indicates fitting of data to a 1:1 binding model.

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Product Information

IL6Cys1 is an unconjugated recombinant anti IL6 Nanobody (VHH). Suitable for for dual cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteines reduced.

• Applications: Conjugation
• Reactivity: Human
• Conjugate: Unconjugated
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity: 3 nM
• Molecular Weight: 13.7 kDa
• Form: Liquid
• RRID: AB_3665393
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 0.09% sodium azide
• Storage Condition: Store at -20°C
• Shipping: dry ice
• Background: Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.