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ChromoTek anti HA-Label, CoraLite ® Plus 555

Bivalent anti-HA-Tag VHH (Nanobody) that is conjugated to fluorescent dyes for immunofluorescence, microscopy, and immunoblotting of HA-tagged proteins.

Host/Type

Alpaca

Reactivity

HA Tag

Applications

IF, WB

Conjugate

CoraLite ® Plus 555

Cat no : hbCL555

Synonyms

HA VHH, HA single domain antibody, HA Label

Validation Data Gallery

  • Immunofluorescence with anti-HA-Label, CoraLite ® Plus 555 (hbCL555, 1:500, orange) visualizes HA-tagged Vimentin, transiently expressed in HeLa cells. Nuclei are stained with DAPI (cyan). Confocal images were were acquired with a 63x oil objective and post-processed.

    Immunofluorescence with anti-HA-Label, CoraLite ® Plus 555 (hbCL555, 1:500, orange) visualizes HA-tagged Vimentin, transiently expressed in HeLa cells. Nuclei are stained with DAPI (cyan). Confocal images were were acquired with a 63x oil objective and post-processed.

  • Multiplex Western Blot with HA-Label (hbCL555, 1:500) targeting HA-labeled TOM70 (blue) and CoraLite®647-conjugated GAPDH (Human Specific) Recombinant antibody (red) 1:500; PTG CL647-80570. Tested on HEK293T lysate and HEK293T transfected with TOM70-HA (Protein ladder: PL00002)

    Multiplex Western Blot with HA-Label (hbCL555, 1:500) targeting HA-labeled TOM70 (blue) and CoraLite®647-conjugated GAPDH (Human Specific) Recombinant antibody (red) 1:500; PTG CL647-80570. Tested on HEK293T lysate and HEK293T transfected with TOM70-HA (Protein ladder: PL00002)

Product Information

Bivalent anti-HA-Tag VHH (Nanobody) that is conjugated to fluorescent dyes for immunofluorescence, microscopy, and immunoblotting of HA-tagged proteins.

DescriptionHA-Label is much smaller compared to conventional primary plus secondary IgG antibodies (IgG complex). Due to its small size, HA-Label is optimal for effective labeling with minimal epitope-label displacement for imaging applications, escpecially super-resolution microscopy. The small size further leads to superior accessibility and labelling of epitopes in crowded cellular/organelle environments. High affinity due to strong avidity effect from bivalent format of the HA-Label.
ApplicationsIF, WB
ReactivityHA Tag
ConjugateCoraLite ® Plus 555
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity1:1 affinity 6 nM C-term. HA, 180 nM N-term. HA. But higher in IF and WB owing to its bivalent format and hence avidity effects
Molecular Weight28.5 kDa
FormLiquid
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
RRIDAB_3665418
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, 0.09% sodium azide
Storage condition-20°C
Shippingblue ice