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ChromoTek anti HA-Label, CoraLite ® Plus 488

Host/Type

Alpaca

Reactivity

HA Tag

Applications

IF, WB

Conjugate

CoraLite ® Plus 488

Cat no : hbCL488

Synonyms

HA VHH, HA single domain antibody, HA Label

Validation Data Gallery

  • Immunofluorescence with anti-HA-Label, CoraLite ® Plus 488 (hbCL488, 1:500, green) visualizes HA-tagged Vimentin, transiently expressed in HeLa cells. Nuclei are stained with DAPI (blue). Confocal images were were acquired with a 63x oil objective and post-processed.

    Immunofluorescence with anti-HA-Label, CoraLite ® Plus 488 (hbCL488, 1:500, green) visualizes HA-tagged Vimentin, transiently expressed in HeLa cells. Nuclei are stained with DAPI (blue). Confocal images were were acquired with a 63x oil objective and post-processed.

  • Multiplex Western Blot with HA-Label (hbCL488, 1:500) targeting HA-labeled TOM70 (blue) and CoraLite®647-conjugated GAPDH (Human Specific) Recombinant antibody (red) 1:500; PTG CL647-80570. Tested on HEK293T lysate and HEK293T transfected with TOM70-HA (Protein ladder: PL00002)

    Multiplex Western Blot with HA-Label (hbCL488, 1:500) targeting HA-labeled TOM70 (blue) and CoraLite®647-conjugated GAPDH (Human Specific) Recombinant antibody (red) 1:500; PTG CL647-80570. Tested on HEK293T lysate and HEK293T transfected with TOM70-HA (Protein ladder: PL00002)

Product Information

ApplicationsIF, WB
ReactivityHA Tag
ConjugateCoraLite ® Plus 488
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity1:1 affinity 6 nM C-term. HA, 180 nM N-term. HA. But higher in IF and WB owing to its bivalent format and hence avidity effects
Molecular Weight28.5 kDa
FormLiquid
Excitation/Emission Maxima Wavelengths500 nm / 520 nm
RRIDAB_3665417
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, 0.09% sodium azide
Storage condition-20°C
Shippingblue ice
Backgroundanti-HA-Tag VHH / Nanobody that is conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of HA-tagged proteins. HA-Label is much smaller compared to conventional primary plus secondary IgG antibodies (IgG complex). Due to its small size, HA-Label is optimal for effective labeling with minimal epitope-label displacement for imaging applications, escpecially super-resolution microscopy. The small size further leads to superior accessibility and labelling of epitopes in crowded cellular/organelle environments.