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ChromoTek Nano-Secondary® alpaca anti-mouse IgG3, recombinant VHH, Alexa Fluor® 647 [CTK0107]

Nano-Secondary® alpaca anti-mouse IgG3, Fab-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of a Nanobody that binds to mouse IgG3 with high affinity & specificity.

Applications

IF, WB

Conjugate

Alexa Fluor® 647

Host

Alpaca

Reactivity

Mouse IgG3

Cat no : sms3AF647-1

Synonyms

mouse IgG3; mouse immunoglobulin Gamma 3

Validation Data Gallery

  • Anti-mouse IgG3 Nano-Secondary: Well-defined and characterized immunostaining. Primary anti-mouse IgG3 antibody (grey) with 2 copies of a monoclonal mouse Fab- specific Nanobody (green) bound. In total, 6 fluorophores (red stars) label the mouse IgG3 primary antibody.

    Anti-mouse IgG3 Nano-Secondary: Well-defined and characterized immunostaining. Primary anti-mouse IgG3 antibody (grey) with 2 copies of a monoclonal mouse Fab- specific Nanobody (green) bound. In total, 6 fluorophores (red stars) label the mouse IgG3 primary antibody.

  • HeLa cells were immunostained with mouse IgG3 anti-Lamin A/C antibody + alpaca anti-mouse IgG3 VHH Alexa Fluor® 647 (magenta). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    HeLa cells were immunostained with mouse IgG3 anti-Lamin A/C antibody + alpaca anti-mouse IgG3 VHH Alexa Fluor® 647 (magenta). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • One-step staining (left) vs. sequential staining (right) of HeLa cells with anti-MOT (mitochondria) mouse IgG3 monoclonal primary antibody + alpaca anti-mouse IgG3 VHH Alexa Fluor® 647 (magenta). Cell nuclei are stained with DAPI (blue). Scale bar, 20 μm.

    One-step staining (left) vs. sequential staining (right) of HeLa cells with anti-MOT (mitochondria) mouse IgG3 monoclonal primary antibody + alpaca anti-mouse IgG3 VHH Alexa Fluor® 647 (magenta). Cell nuclei are stained with DAPI (blue). Scale bar, 20 μm.

  • Multiplexed immunostaining of HeLa cells with two alpaca anti-mouse Nano-Secondaries and one conventional secondary antibody. Green: mouse IgG3 anti-Lamin + alpaca anti-mouse IgG3 VHH Alexa Fluor® 488. Red: mouse IgG1 anti-Tubulin + alpaca anti-mouse gG1 VHH Alexa Fluor® 568. Magenta: rabbit anti-Ki67 + conventional polyclonal secondary anti-rabbit-AF647. Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Multiplexed immunostaining of HeLa cells with two alpaca anti-mouse Nano-Secondaries and one conventional secondary antibody. Green: mouse IgG3 anti-Lamin + alpaca anti-mouse IgG3 VHH Alexa Fluor® 488. Red: mouse IgG1 anti-Tubulin + alpaca anti-mouse gG1 VHH Alexa Fluor® 568. Magenta: rabbit anti-Ki67 + conventional polyclonal secondary anti-rabbit-AF647. Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Higher resolution with anti-mouse IgG3 Nano-Secondary compared to conventional secondary antibodies. Left: Formation of a small, precise complex of Nanobodies (green) & primary antibody (grey). Right: Formation of a large, arbitrary complex of multiple polyclonal secondaries (green) & primary antibody.

    Higher resolution with anti-mouse IgG3 Nano-Secondary compared to conventional secondary antibodies. Left: Formation of a small, precise complex of Nanobodies (green) & primary antibody (grey). Right: Formation of a large, arbitrary complex of multiple polyclonal secondaries (green) & primary antibody.

Product Information

Nano-Secondary® alpaca anti-mouse IgG3, Fab-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of a Nanobody that binds to mouse IgG3 with high affinity & specificity.

DescriptionNano-Secondary® alpaca anti-mouse IgG3, recombinant VHH reagent uses a novel class of anti-mouse IgG3 isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG3 with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your experimental. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below.
ApplicationsIF, WB
HostAlpaca
Species ReactivityMouse IgG3 Fc-fragment
No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG1, IgG2a, IgG2b, IgG2c
Target/SpecificityThis Nanobody mixture recognizes the Fab fragment of mouse IgG3.
Physical StateLiquid
ConjugateAlexa Fluor® 647
Suggested DilutionImmunofluorescence 1:1,000
Western blot 1:1,000
Purification MethodRecombinant expression, affinity purification IMAC
TypeNanobody
ClassRecombinant
FormatAlpaca single domain antibodies, monovalent
Cross-reactivityNo cross-reactivity to rabbit, rat, sheep, goat, guinea pig, human, and macaque serum
ImmunogenPurified mouse IgG3
ClonalityMonoclonal
ClonesVHH0283
RRIDAB_2827584
Affinity (KD)CTK0107: KD = 5.7 nM
Excitation / EmissionExcitation max: 650 nm, Emission max: 665 nm
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
Storage ConditionStore at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles.
Size10 µL; 100 µL
NoteThis product is for research use only, not for diagnostic or therapeutic use