ZNF75D Polyclonal antibody

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• KD/KO Validated

ZNF75D Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21583-1-AP

Synonyms

D8C6, Zinc finger protein 75, zinc finger protein 75D, Zinc finger protein 82, ZNF75, ZNF75D, ZNF82

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Validation Data Gallery

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  WB analysis using 21583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21583-1-AP (ZNF75D antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 21583-1-AP

  WB result of ZNF75D antibody (21583-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF75D transfected RAW 264.7 cells.

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Tested Applications

• Positive WB detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21583-1-AP targets ZNF75D in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF75D fusion protein Ag16139
• Full Name: zinc finger protein 75D
• Calculated Molecular Weight: 510 aa, 59 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC109100
• Gene Symbol: ZNF75D
• Gene ID (NCBI): 7626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF75D (zinc finger protein 75D), also known as ZNF75. It is expected to be located in nucleus and ubiquitous expression in prostate and adrenal. The calculated molecular weight of METAP1 is 60 kDa. The gene encodes a protein that likely functions as a transcription factor. ZNF75D belongs to the ZNF75 family, includes an N-terminal SCAN domain, a KRAB box, and five C2H2-type zinc finger motifs. Another functional gene belonging to this family is located on chromosome 16, while pseudogenes have been identified on chromosomes 11 and 12 (PMID: 8661144). It may be involved in transcriptional regulation.