ZNF497 Polyclonal antibody

ZNF497 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25178-1-AP

Synonyms

zinc finger protein 497, ZNF497

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Validation Data Gallery

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  WB analysis of mouse testis using 25178-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25178-1-AP (ZNF497 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 25178-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25178-1-AP (ZNF497 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 25178-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25178-1-AP (ZNF497 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 25178-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25178-1-AP (ZNF497 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 25178-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25178-1-AP (ZNF497 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: human kidney tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25178-1-AP is ZNF497 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF497 fusion protein Ag18114
• Full Name: zinc finger protein 497
• Calculated Molecular Weight: 498 aa, 55 kDa
• Observed Molecular Weight: 61-65 kDa
• GenBank Accession Number: BC118983
• Gene Symbol: ZNF497
• Gene ID (NCBI): 162968
• RRID: AB_2879944
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.