ZNF202 Polyclonal antibody

ZNF202 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26216-1-AP

Synonyms

ZKSCAN10, Zinc finger protein 202

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Validation Data Gallery

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  WB analysis using 26216-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26216-1-AP (ZNF202 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26216-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26216-1-AP (ZNF202 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spermatophore using 26216-1-AP

  mouse spermatophore tissue were subjected to SDS PAGE followed by western blot with 26216-1-AP (ZNF202 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 26216-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26216-1-AP (ZNF202 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HL-60 cells, human testis tissue, mouse spermatophore tissue, HeLa cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26216-1-AP targets ZNF202 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF202 fusion protein Ag23859
• Full Name: zinc finger protein 202
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC013382
• Gene Symbol: ZNF202
• Gene ID (NCBI): 7753
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF202 gene product is a transcriptional repressor that binds to elements found predominantly in genes that participate in lipid metabolism. Among its targets are structural components of lipoprotein particles (apolipoproteins AIV, CIII, and E), enzymes involved in lipid processing (lipoprotein lipase, lecithin cholesteryl ester transferase), and several genes involved in processes related to energy metabolism and vascular disease (PMID: 10748193). ZNF202 is expressed in two common splice variants. The α protein consists of 424 amino acids, while the β-protein consists of 648 amino acids (PMID: 11279031).