ZNF184 Polyclonal antibody

ZNF184 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26100-1-AP

Synonyms

zinc finger protein 184, ZNF184

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 26100-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26100-1-AP (ZNF184 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 26100-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26100-1-AP (ZNF184 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 26100-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26100-1-AP (ZNF184 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 26100-1-AP

  Immunofluorescent analysis of HepG2 cells using 26100-1-AP (ZNF184 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, MCF-7 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26100-1-AP is ZNF184 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF184 fusion protein Ag23314
• Full Name: zinc finger protein 184
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC022992
• Gene Symbol: ZNF184
• Gene ID (NCBI): 7738
• RRID: AB_2880376
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.