ZNF143 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZNF143 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 16618-1-AP

Synonyms

hStaf, pHZ 1, SBF, SPH binding factor, STAF, zinc finger protein 143, ZNF143

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Validation Data Gallery

• 

  WB analysis of HepG2 using 16618-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16618-1-AP (ZNF143 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16618-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16618-1-AP (ZNF143 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16618-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16618-1-AP (ZNF143 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 8 publications below

Product Information

16618-1-AP targets ZNF143 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF143 fusion protein Ag9914
• Full Name: zinc finger protein 143
• Calculated Molecular Weight: 626 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC020219
• Gene Symbol: ZNF143
• Gene ID (NCBI): 7702
• RRID: AB_2218324
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger protein143(ZNF143) contains a separete acidic activation and 7 zinc finger domains. It's a transcription factor that required for activation of the majority of vertebrate snRNA and snRNA-type genes, which transcribed by RNA pol II and pol III. Also, it binds to the SPH motif of small nuclear RNA (snRNA) gene promoters and participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8

Publications

Species	Application	Title
mouse	chIP	Mol Cell; Maintenance of CTCF- and Transcription Factor-Mediated Interactions from the Gametes to the Early Mouse Embryo.; Authors - Yoon Hee Jung
human	chIP	Mol Cell; Architectural Proteins and Pluripotency Factors Cooperate to Orchestrate the Transcriptional Response of hESCs to Temperature Stress.; Authors - Xiaowen Lyu
mouse	CoIP	Nat Commun; Mutations in Hcfc1 and Ronin result in an inborn error of cobalamin metabolism and ribosomopathy.; Authors - Tiffany Chern
human	ChIP	Proc Natl Acad Sci U S A; Cooperative transcription factor associations discovered using regulatory variation.; Authors - Karczewski Konrad J KJ
human	chIP	Cancer Res; ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma.; Authors - Lili Zhang ; KD Validated
human	WB, ChIP	Cell Rep; Host Cell Factor-1 Recruitment to E2F-Bound and Cell-Cycle-Control Genes Is Mediated by THAP11 and ZNF143.; Authors - J Brandon Parker ; KD Validated