ZMYND10 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZMYND10 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14431-1-AP

Synonyms

BLU, FLU, Protein BLu, ZMYND10

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Validation Data Gallery

• 

  WB analysis using 14431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14431-1-AP (ZMYND10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 14431-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14431-1-AP (ZMYND10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 14431-1-AP

  IP result of anti-ZMYND10 (IP:14431-1-AP, 4ug; Detection:14431-1-AP 1:500) with SH-SY5Y cells lysate 1600ug.

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Tested Applications

• Positive WB detected in: human testis tissue, SH-SY5Y cells, mouse testis tissue, rat testis tissue
• Positive IP detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

14431-1-AP targets ZMYND10 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZMYND10 fusion protein Ag5808
• Full Name: zinc finger, MYND-type containing 10
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC033732
• Gene Symbol: ZMYND10
• Gene ID (NCBI): 51364
• RRID: AB_2218002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF,IP	Elife; ZMYND10 functions in a chaperone relay during axonemal dynein assembly.; Authors - Girish R Mali ; KO Validated
human	WB,IF	Andrology; A novel homozygous LRRC6 mutation causes male infertility with asthenozoospermia and primary ciliary dyskinesia in humans; Authors - Shengjia Shi
rat	WB	Environ Toxicol; Sodium arsenite impairs sperm quality via downregulating the ZMYND15 and ZMYND10; Authors - Xiangli Li