ZHX1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZHX1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13903-1-AP

Synonyms

ZHX1, zinc fingers and homeoboxes 1

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Validation Data Gallery

• 

  WB analysis of HeLa using 13903-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13903-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13903-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13903-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13903-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13903-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 13903-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 13903-1-AP

  IP result of anti-ZHX1 (IP:13903-1-AP, 4ug; Detection:13903-1-AP 1:500) with HEK-293 cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, mouse heart tissue, mouse kidney tissue
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

13903-1-AP targets ZHX1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZHX1 fusion protein Ag4874
• Full Name: zinc fingers and homeoboxes 1
• Calculated Molecular Weight: 873 aa, 98 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC040481
• Gene Symbol: ZHX1
• Gene ID (NCBI): 11244
• RRID: AB_2219214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Binding of the nuclear factor-Y complex (NF-Y) to the inverted CCAAT-box interferes with transcription activation through nucleosome reorganization. The three homologous proteins forming the zinc-fingers and homeoboxes (ZHX) family interact with the activation domain of NF-Ya to repress transcription [PMID:19348505,20509910]. Human zinc-fingers and homeoboxes 1 (ZHX1) was cloned as a protein that interacts with the activation domain (AD) of the A subunit of nuclear factor-Y (NF-YA) [PMID:12237128].

Publications

Species	Application	Title
human	WB,IHC	PLoS One; ZHX1 Promotes the Proliferation, Migration and Invasion of Cholangiocarcinoma Cells.; Authors - Ryuk-Jun Kwon ; KD Validated
human	WB,IHC	Tumour Biol; Roles of zinc-fingers and homeoboxes 1 during the proliferation, migration, and invasion of glioblastoma cells.; Authors - Ryuk-Jun Kwon ; KD Validated
human	WB	Mol Ther Nucleic Acids; Blocking lncRNA MALAT1/miR-199a/ZHX1 Axis Inhibits Glioblastoma Proliferation and Progression.; Authors - Keman Liao