Protrudin Polyclonal antibody

• Featured Product
• KD/KO Validated

Protrudin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 12680-1-AP

Synonyms

ZFYVE27, Zinc finger FYVE domain-containing protein 27, SPG33, Spastic paraplegia 33 protein, RP11 459F3.2

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Validation Data Gallery

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  WB analysis of mouse thymus using 12680-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12680-1-AP (Protrudin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12680-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12680-1-AP (Protrudin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12680-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12680-1-AP (Protrudin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12680-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12680-1-AP (Protrudin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 12680-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 12680-1-AP (Protrudin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse thymus tissue, mouse testis tissue, mouse brain tissue, Jurkat cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IF: See 4 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

12680-1-AP targets Protrudin in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Protrudin fusion protein Ag3369
• Full Name: zinc finger, FYVE domain containing 27
• Calculated Molecular Weight: 411 aa, 46 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC030621
• Gene Symbol: ZFYVE27
• Gene ID (NCBI): 118813
• RRID: AB_10640298
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protrudin, also named as ZFYVE27, is a 411 amino acid protein, which contains 1 FYVE-type zinc finger. Protrudin localizes in the recycling endosome membrane and functions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. Protrudin is a key regulator of vesicular transport during neurite extension and is involved in neuronal intracellular trafficking in the corticospinal tract.

Publications

Species	Application	Title
human	WB,IP	Nat Commun; PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria.; Authors - Yael Elbaz-Alon
rat	WB	Nat Commun; Protrudin functions from the endoplasmic reticulum to support axon regeneration in the adult CNS.; Authors - Veselina Petrova
human	WB	Nat Commun; Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry.; Authors - Parikshit Bagchi
human	WB,IF,IP	Proc Natl Acad Sci U S A; Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.; Authors - Chang Jaerak J ; KD Validated
human		Cell Mol Life Sci; ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic.; Authors - James W Connell
human	WB	Cell Mol Life Sci; Protrudin regulates FAK activation, endothelial cell migration and angiogenesis.; Authors - Amita Arora ; KD Validated