Protrudin Polyclonal antibody

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• KD/KO Validated

Protrudin Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12680-1-AP Copied!

Publications (16)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

ZFYVE27, RP11 459F3.2, Spastic paraplegia 33 protein, SPG33, Zinc finger FYVE domain-containing protein 27

• 

  WB analysis of mouse thymus using 12680-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12680-1-AP (Protrudin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 12680-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12680-1-AP (Protrudin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 12680-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12680-1-AP (Protrudin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse testis using 12680-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12680-1-AP (Protrudin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 12680-1-AP

  IP result of anti-Protrudin (IP:12680-1-AP, 4ug; Detection:12680-1-AP 1:600) with mouse brain tissue lysate 1680 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 12680-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 12680-1-AP (Protrudin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse thymus tissue, mouse testis tissue, mouse brain tissue, Jurkat cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12680-1-AP targets Protrudin in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3369

  Product name: Recombinant human ZFYVE27 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 217-411 aa of BC030621

  Sequence: VEFFRVVSEYRASLQQRMNPKQEEHAFESPPPPDVGGKDGLMDSTPALTPTEDLTPGSVEEAEEAEPDEEFKDAIEETHLVVLEDDEGAPCPAEDELALQDNGFLSKNEVLRSKVSRLTERLRKRYPTNNFGNCTGCSATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK

• Full Name: zinc finger, FYVE domain containing 27
• Calculated Molecular Weight: 411 aa, 46 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC030621
• Gene Symbol: ZFYVE27
• Gene ID (NCBI): 118813
• RRID: AB_10640298
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5T4F4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protrudin, also named as ZFYVE27, is a 411 amino acid protein, which contains 1 FYVE-type zinc finger. Protrudin localizes in the recycling endosome membrane and functions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. Protrudin is a key regulator of vesicular transport during neurite extension and is involved in neuronal intracellular trafficking in the corticospinal tract.

Publications

What published studies show

The Protrudin antibody (Cat# 12680-1-AP) has 16 total citations across 12 journals, including Nature Communications, Nature Methods, PNAS, Cell Reports, and Cell Molecular Life Sciences. Research fields span endoplasmic reticulum morphology and membrane contact sites, axon regeneration, synaptic plasticity, viral entry, cholesterol metabolism, seizure regulation, phagocytosis, angiogenesis, endosomal trafficking, lysosome positioning, and hereditary spastic paraplegia pathogenesis.

Automatically generated from the 16 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Signal Transduct Target Ther; Targeting intracellular cholesterol imbalance rescues sarcomere-ER contact site signaling and ER remodeling in dilated cardiomyopathy.; Authors - Nadezda Ignatyeva
monkey	WB	Nat Methods; ERnet: a tool for the semantic segmentation and quantitative analysis of endoplasmic reticulum topology; Authors - Meng Lu ; KD Validated
rat	WB	Nat Commun; Protrudin functions from the endoplasmic reticulum to support axon regeneration in the adult CNS.; Authors - Veselina Petrova
human	WB,IP	Nat Commun; PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria.; Authors - Yael Elbaz-Alon
human	WB	Nat Commun; Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry.; Authors - Parikshit Bagchi
		Redox Biol; Glucocorticoid enhances presenilin1-dependent Aβ production at ER's mitochondrial-associated membrane by downregulating Rer1 in neuronal cells; Authors - Gee Euhn Choi