|Positive WB detected in||mouse thymus tissue, mouse testis tissue, mouse brain tissue, Jurkat cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Sample-dependent, check data in validation data gallery|
12680-1-AP targets Protrudin in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Protrudin fusion protein Ag3369|
|Full Name||zinc finger, FYVE domain containing 27|
|Calculated molecular weight||411 aa, 46 kDa|
|Observed molecular weight||46-50 kDa|
|GenBank accession number||BC030621|
|Gene ID (NCBI)||118813|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Protrudin, also named as ZFYVE27, is a 411 amino acid protein, which contains 1 FYVE-type zinc finger. Protrudin localizes in the recycling endosome membrane and functions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. Protrudin is a key regulator of vesicular transport during neurite extension and is involved in neuronal intracellular trafficking in the corticospinal tract.
PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria.
Protrudin functions from the endoplasmic reticulum to support axon regeneration in the adult CNS.
Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry.
Proc Natl Acad Sci U S A
Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.
Cell Mol Life Sci
ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic.
Cell Mol Life Sci
Protrudin regulates FAK activation, endothelial cell migration and angiogenesis.