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Protrudin Polyclonal antibody
Protrudin Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 12680-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse thymus tissue, mouse testis tissue, mouse brain tissue, Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 4 publications below |
WB | See 11 publications below |
IF | See 4 publications below |
IP | See 3 publications below |
CoIP | See 1 publications below |
Product Information
12680-1-AP targets Protrudin in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, monkey |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Protrudin fusion protein Ag3369 |
Full Name | zinc finger, FYVE domain containing 27 |
Calculated Molecular Weight | 411 aa, 46 kDa |
Observed Molecular Weight | 46-50 kDa |
GenBank Accession Number | BC030621 |
Gene Symbol | ZFYVE27 |
Gene ID (NCBI) | 118813 |
RRID | AB_10640298 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protrudin, also named as ZFYVE27, is a 411 amino acid protein, which contains 1 FYVE-type zinc finger. Protrudin localizes in the recycling endosome membrane and functions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. Protrudin is a key regulator of vesicular transport during neurite extension and is involved in neuronal intracellular trafficking in the corticospinal tract.
Protocols
Product Specific Protocols | |
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WB protocol for Protrudin antibody 12680-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria. | ||
Nat Commun Protrudin functions from the endoplasmic reticulum to support axon regeneration in the adult CNS. | ||
Nat Commun Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry. | ||
Proc Natl Acad Sci U S A Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.
| ||
Cell Mol Life Sci ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic. | ||
Cell Mol Life Sci Protrudin regulates FAK activation, endothelial cell migration and angiogenesis.
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