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  • KD/KO Validated

Protrudin Polyclonal antibody

Protrudin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12680-1-AP

Synonyms

PROTRUDIN, RP11 459F3.2, SPG33, ZFYVE27



Tested Applications

Positive WB detected inmouse thymus tissue, mouse testis tissue, mouse brain tissue, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12680-1-AP targets Protrudin in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Protrudin fusion protein Ag3369
Full Name zinc finger, FYVE domain containing 27
Calculated Molecular Weight 411 aa, 46 kDa
Observed Molecular Weight 46-50 kDa
GenBank Accession NumberBC030621
Gene Symbol ZFYVE27
Gene ID (NCBI) 118813
RRIDAB_10640298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protrudin, also named as ZFYVE27, is a 411 amino acid protein, which contains 1 FYVE-type zinc finger. Protrudin localizes in the recycling endosome membrane and functions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. Protrudin is a key regulator of vesicular transport during neurite extension and is involved in neuronal intracellular trafficking in the corticospinal tract.

Protocols

Product Specific Protocols
WB protocol for Protrudin antibody 12680-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Nat Commun

PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria.

Authors - Yael Elbaz-Alon
ratWB

Nat Commun

Protrudin functions from the endoplasmic reticulum to support axon regeneration in the adult CNS.

Authors - Veselina Petrova
humanWB

Nat Commun

Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry.

Authors - Parikshit Bagchi
humanWB,IF,IP

Proc Natl Acad Sci U S A

Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.

Authors - Chang Jaerak J
  • KD Validated
human

Cell Mol Life Sci

ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic.

Authors - James W Connell
humanWB

Cell Mol Life Sci

Protrudin regulates FAK activation, endothelial cell migration and angiogenesis.

Authors - Amita Arora
  • KD Validated