ZFP64 Recombinant antibody

ZFP64 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240847F9

Cat no : 83825-3-RR

Synonyms

ZNF338, Zinc finger protein 64, Zfp-64, Zfp 64, 240847F9

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Validation Data Gallery

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  WB analysis using 83825-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83825-3-RR (ZFP64 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83825-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83825-3-RR against Human ZFP64 were performed. The affinity constant is 1.53 nM.

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Tested Applications

• Positive WB detected in: K-562 cells, Jurkat cells, MKN-45 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83825-3-RR is ZFP64 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZFP64 fusion protein Ag10971
• Full Name: zinc finger protein 64 homolog (mouse)
• Calculated Molecular Weight: 681 aa, 75 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC041622
• Gene Symbol: ZFP64
• Gene ID (NCBI): 55734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZFP64 (also known as ZNF338) is a member of the Krüppel C2H2-type zinc finger family. ZFP64 is a coactivator of Notch1, mediating mesenchymal cell differentiation. ZFP64 expression was up-regulated in mouse peritoneal macrophages in response to stimulation with the TLR4 ligand LPS with a 2-fold increase. The MLL gene encoding the MLL fusion protein is easily activated by ZFP64. ZFP64 is essential for the continuous production of MLL fusion proteins in blood cancer and produces more cancer cells. Since ZFP64 deletion completely and specifically inhibits MLL fusion protein production and leukemia cascade growth, it is an ideal drug target.