ZFP64 Polyclonal antibody

ZFP64 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, chIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17187-1-AP

Synonyms

Zfp 64, ZFP64, Zinc finger protein 338, ZNF338

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Validation Data Gallery

• 

  WB analysis of mouse spleen using 17187-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17187-1-AP (ZFP64 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17187-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17187-1-AP (ZFP64 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse spleen tissue, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below
• ChIP: See 2 publications below

Product Information

17187-1-AP targets ZFP64 in WB, IF, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZFP64 fusion protein Ag10971
• Full Name: zinc finger protein 64 homolog (mouse)
• Calculated Molecular Weight: 681 aa, 75 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC041622
• Gene Symbol: ZFP64
• Gene ID (NCBI): 55734
• RRID: AB_2218826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZFP64 (also known as ZNF338) is a member of the Krüppel C2H2-type zinc finger family. ZFP64 is a coactivator of Notch1, mediating mesenchymal cell differentiation. ZFP64 expression was up-regulated in mouse peritoneal macrophages in response to stimulation with the TLR4 ligand LPS with a 2-fold increase. The MLL gene encoding the MLL fusion protein is easily activated by ZFP64. ZFP64 is essential for the continuous production of MLL fusion proteins in blood cancer and produces more cancer cells. Since ZFP64 deletion completely and specifically inhibits MLL fusion protein production and leukemia cascade growth, it is an ideal drug target.

Publications

Species	Application	Title
human	chIP	Cancer Cell; A Transcription Factor Addiction in Leukemia Imposed by the MLL Promoter Sequence.; Authors - Bin Lu
mouse	IF	Nat Neurosci; Neuronal activity rapidly reprograms dendritic translation via eIF4G2:uORF binding; Authors - Ezgi Hacisuleyman
human	WB,ChIP	Biol Direct; ZFP64 drives glycolysis-mediated stem cell-like properties and tumorigenesis in breast cancer; Authors - Jiayi Sun