ZC3HAV1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZC3HAV1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

31

Reviews (1)

Cat no : 16820-1-AP

Synonyms

FLB6421, ZAP, ZC3H2, ZC3HAV1, ZC3HDC2, Zinc finger antiviral protein

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Validation Data Gallery

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  WB analysis using 16820-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16820-1-AP (ZC3HAV1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16820-1-AP

  WB result of ZC3HAV1 antibody (16820-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZC3HAV1 transfected HEK-293 cells.

• 

  WB analysis of HeLa using 16820-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16820-1-AP (ZC3HAV1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16820-1-AP

  IP result of anti-ZC3HAV1 (IP:16820-1-AP, 4ug; Detection:16820-1-AP 1:2000) with HeLa cells lysate 1080ug.

• 

  IHC staining of human kidney using 16820-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16820-1-AP (ZC3HAV1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16820-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16820-1-AP (ZC3HAV1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 16820-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 16820-1-AP (ZC3HAV1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 11 publications below
• WB: See 26 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 4 publications below
• RIP: See 1 publications below

Product Information

16820-1-AP targets ZC3HAV1 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZC3HAV1 fusion protein Ag10364
• Full Name: zinc finger CCCH-type, antiviral 1
• Calculated Molecular Weight: 699aa,78 kDa; 902aa,101 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC025308
• Gene Symbol: ZC3HAV1
• Gene ID (NCBI): 56829
• RRID: AB_2728733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms. ZC3HAV1 exists some isoforms with MV 101, 77, 67, 42 and 18 kDa.

Publications

Species	Application	Title
human	WB	Nature; CG dinucleotide suppression enables antiviral defence targeting non-self RNA.; Authors - Matthew A Takata ; KD Validated
human	WB	Cell; SCAF4 and SCAF8, mRNA Anti-Terminator Proteins.; Authors - Lea H Gregersen
		Cell Host Microbe; HIV-1 matrix-tRNA complex structure reveals basis for host control of Gag localization.; Authors - Charles Bou-Nader
human	WB	Nat Microbiol; Rational attenuation of RNA viruses with zinc finger antiviral protein; Authors - Daniel Gonçalves-Carneiro
human	WB,IP	Nat Commun; The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting.; Authors - Matthias M Zimmer
human	WB	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (10-26-2022)

This is a fantastic antibody. It works very reliably at high dilutions.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T, HELA, MT4, SupT1

ZC3HAV1 Antibody Western Blot validation (1:5000 dilution) in HEK293T, HELA, MT4, SupT1 (Cat no:16820-1-AP)

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