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- KD/KO Validated
YTHDF2 Polyclonal antibody
YTHDF2 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, RIP, IP, IHC, IF, FC, CoIP, CLIP, chIP, ELISA
Conjugate
Unconjugated
212
Cat no : 24744-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, Jurkat cells, HeLa cells, MOLT-4 cells, NIH/3T3 cells, C6 cells, Raji cells |
Positive IP detected in | Jurkat cells |
Positive IF detected in | mouse brain tissue, U2OS cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
24744-1-AP targets YTHDF2 in WB, RIP, IP, IHC, IF, FC, CoIP, CLIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, monkey, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | YTHDF2 fusion protein Ag5922 |
Full Name | YTH domain family, member 2 |
Calculated molecular weight | 62 kDa |
Observed molecular weight | 62 kDa |
GenBank accession number | BC002559 |
Gene symbol | YTHDF2 |
Gene ID (NCBI) | 51441 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
YTHDF2 also named as CLL associated antigen KW 14 or HGRG8 is a 579 amino acid protein, which contains one YTH domain and exists as two alternatively spliced isoforms. YTHDF2 is expressed in in pancreas, testis and placenta. YTHDF2 may play a role in human longevity.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for YTHDF2 antibody 24744-1-AP | Download protocol |
IF protocol for YTHDF2 antibody 24744-1-AP | Download protocol |
IP protocol for YTHDF2 antibody 24744-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature Dynamic m(6)A mRNA methylation directs translational control of heat shock response. | ||
Cell G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. | ||
Nat Med Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Jinyun (Verified Customer) (08-26-2022) | I did the western blot, and the antibody worked quite well.
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FH Robert (Verified Customer) (01-24-2019) | 1. scrambled siRNA2. YTHDF2 siRNA
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