YTHDF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

YTHDF1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

241

Reviews (8)

Cat no : 17479-1-AP

Synonyms

YTH domain-containing family protein 1, DF1, DACA-1, C20orf21

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Validation Data Gallery

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  WB analysis of HepG2 using 17479-1-AP

  WB result of YTHDF1 antibody (17479-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDF1 transfected HepG2 cells.

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  WB analysis using 17479-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17479-1-AP (YTHDF1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17479-1-AP (YTHDF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17479-1-AP (YTHDF1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17479-1-AP

  IP result of anti-YTHDF1 (IP:17479-1-AP, 4ug; Detection:17479-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

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  IHC staining of human kidney using 17479-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17479-1-AP (YTHDF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17479-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17479-1-AP (YTHDF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hypothalamus using 17479-1-AP

  Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using 17479-1-AP (YTHDF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 17479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using YTHDF1 antibody (17479-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 17479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 17479-1-AP (YTHDF1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 17479-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using YTHDF1 antibody (17479-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, A549 cells, mouse brain tissue, rat brain tissue, HEK-293 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue, human hypothalamus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 79 publications below
• WB: See 209 publications below
• IHC: See 36 publications below
• IF: See 36 publications below
• IP: See 8 publications below
• FC: See 1 publications below
• CoIP: See 4 publications below
• RIP: See 55 publications below

Product Information

17479-1-AP targets YTHDF1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey, chicken, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YTHDF1 fusion protein Ag11509
• Full Name: YTH domain family, member 1
• Calculated Molecular Weight: 559 aa, 61 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC050284
• Gene Symbol: YTHDF1
• Gene ID (NCBI): 54915
• RRID: AB_2217473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YTHDF1, also named YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at the internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing, and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoforms and the calculated molecular weight of isoforms are 61 kDa and 21 kDa.

Publications

Species	Application	Title
human	IF	Nature; m6A enhances the phase separation potential of mRNA.; Authors - Ryan J Ries
mouse	WB	Nature; m6A RNA methylation regulates the fate of endogenous retroviruses.; Authors - Tomasz Chelmicki
mouse	WB,IF	Nature; m6A facilitates hippocampus-dependent learning and memory through YTHDF1.; Authors - Hailing Shi ; KO Validated
human	WB	Cell; G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.; Authors - Peiguo Yang
human	WB	Cell; A Unified Model for the Function of YTHDF Proteins in Regulating m6A-Modified mRNA.; Authors - Sara Zaccara ; KD Validated
mouse	WB	Cell; The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.; Authors - Deniz Simsek

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

RASHMI (Verified Customer) (11-27-2024)

Works great in WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK-293

FH

Fanpeng (Verified Customer) (03-14-2024)

Good for immunostaining and WB analysis using brain tissues and multiple brain cells.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:2000 WB, 1:200 IHC/IF
• Cell Tissue Type: human brain/M17 cell/primary mouse brain cells

FH

Tatyana (Verified Customer) (12-04-2023)

Works well in WB and also gives a cytoplasmic signal in ICC, although it is fairly week. Also works in PLA.

• Applications: Western Blot, Immunofluorescence, Other
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa and SH-SY5Y cells

YTHDF1 Antibody Western Blot,Immunofluorescence,Other validation (1:1000 dilution) in HeLa and SH-SY5Y cells (Cat no:17479-1-AP)

FH

Shinford (Verified Customer) (10-26-2023)

We conducted tests on various m6A-related modulators, and the antibodies related to the YTHDF family consistently performed well. Even at a lower concentration of 1:3000, they yielded satisfactory results.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Cancer cell lines

YTHDF1 Antibody Western Blot validation (1:2000 dilution) in Cancer cell lines (Cat no:17479-1-AP)

FH

Sarah (Verified Customer) (01-05-2021)

Works great in WB at 1/1000 (5% Milk TBS Tween). Tested on HeLa and 293T lysates;

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa and HEL293T

FH

Hannah (Verified Customer) (08-14-2020)

Band observed by WB of correct size that is reduced with siRNA knockdown but required long exposure or Pico supersignal ECL for a good signal from fibroblast lysates

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/1000 for WB; 1/100 for IF
• Cell Tissue Type: human fibroblasts

FH

Biao (Verified Customer) (03-11-2020)

This antibody is very specific and good quality.

• Applications: Western Blot,
• Cell Tissue Type: hct-116

FH

Zizhao (Verified Customer) (12-03-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HaCaT/human keratinocytes

YTHDF1 Antibody Western Blot, validation (1:1000 dilution) in HaCaT/human keratinocytes (Cat no:17479-1-AP)