YTHDC2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

YTHDC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 27779-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 27779-1-AP (same clone as 27779-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27779-1-AP (YTHDC2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27779-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 27779-1-AP (same clone as 27779-1-PBS)

  WB result of YTHDC2 antibody (27779-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDC2 transfected HepG2 cells. This data was developed using the same antibody clone with 27779-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 27779-1-AP (same clone as 27779-1-PBS)

  WB result of YTHDC2 antibody (27779-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDC2 transfected HeLa cells. This data was developed using the same antibody clone with 27779-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 27779-1-AP (same clone as 27779-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 27779-1-AP (YTHDC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27779-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 27779-1-AP (same clone as 27779-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 27779-1-AP (YTHDC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27779-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 27779-1-AP (same clone as 27779-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using YTHDC2 antibody (27779-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 27779-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 27779-1-AP (same clone as 27779-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human YTHDC2 (27779-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 27779-1-PBS in a different storage buffer formulation.

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Product Information

27779-1-PBS targets YTHDC2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YTHDC2 fusion protein Ag26690
• Full Name: YTH domain containing 2
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC137285
• Gene Symbol: YTHDC2
• Gene ID (NCBI): 64848
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

YTHDC2, also named as Probable ATP-dependent RNA helicase YTHDC2, is a 1430 amino acid protein, which belongs to the DEAD box helicase family. YTHDC2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.