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  • KD/KO Validated

YTHDC2 Polyclonal antibody

YTHDC2 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse, rat and More (2)





Cat no : 27779-1-AP


YTH domain containing 2, YTHDC2

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, MCF-7 cells, MDA-MB-231 cells
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27779-1-AP targets YTHDC2 in WB, IP, IF, FC, RIP, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YTHDC2 fusion protein Ag26690
Full Name YTH domain containing 2
Observed Molecular Weight 160 kDa
GenBank Accession NumberBC137285
Gene Symbol YTHDC2
Gene ID (NCBI) 64848
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YTHDC2, also named as Probable ATP-dependent RNA helicase YTHDC2, is a 1430 amino acid protein, which belongs to the DEAD box helicase family. YTHDC2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.


Product Specific Protocols
WB protocol for YTHDC2 antibody 27779-1-APDownload protocol
IHC protocol for YTHDC2 antibody 27779-1-APDownload protocol
IF protocol for YTHDC2 antibody 27779-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cell

Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.

Authors - Hagai Marmor-Kollet

Sci Adv

Regulation of telomere homeostasis and genomic stability in cancer by N 6-adenosine methylation (m6A).

Authors - Ji Hoon Lee

Nat Commun

Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.

Authors - Shujie Chen

Cancer Lett

LIMK1 m6A-RNA methylation recognized by YTHDC2 induces 5-FU chemoresistance in colorectal cancer via endoplasmic reticulum stress and stress granule formation

Authors - Lu Chen
  • KD Validated

Genes Dev

YTHDC2 control of gametogenesis requires helicase activity but not m6A binding.

Authors - Yuhki Saito


Genetic variants in m6A modification genes are associated with esophageal squamous-cell carcinoma in the Chinese population.

Authors - Nan Yang
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Maialen (Verified Customer) (02-10-2022)

YTHDC2 was immunoprecipitated from 4 million HCT15 cells lysate using 1μg 27779-1-AP (YTHDC2 antibody). Same amount of normal rabbit IgG was used as negative control. For IP confirmation, western blot with 27779-1-AP (YTHDC2 antibody) was perfomed at 1:1000 dilution incubated overnight at 4ºC. 10% of the whole cell lysate was loaded as input.

  • Applications: Western Blot, Immunoprecipitation
  • Cell Tissue Type: HCT15
YTHDC2 Antibody Western Blot,Immunoprecipitation validation ( dilution) in HCT15 (Cat no:27779-1-AP)

Robert (Verified Customer) (01-24-2019)

1. scrambled siRNA2. YTHDC2 siRNA12. YTHDC2 siRNA22. YTHDC2 siRNA3

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: ST cells (porcine)
YTHDC2 Antibody Western Blot, validation (1/500 dilution) in ST cells (porcine) (Cat no:27779-1-AP)