YTHDC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

YTHDC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IP, IF, RIP, IHC, ELISA

Conjugate

Unconjugated

Publications

13

Reviews (1)

Cat no : 29441-1-AP

Synonyms

KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1

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Validation Data Gallery

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  WB analysis using 29441-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29441-1-AP (YTHDC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29441-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29441-1-AP (YTHDC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29441-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29441-1-AP (YTHDC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29441-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29441-1-AP (YTHDC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 29441-1-AP

  WB result of YTHDC1 antibody (29441-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDC1 transfected HEK-293 cells.

• 

  WB analysis of HepG2 using 29441-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 29441-1-AP (YTHDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 29441-1-AP

  IP result of anti-YTHDC1 (IP:29441-1-AP, 4ug; Detection:29441-1-AP 1:2000) with HEK-293 cells lysate 1800 ug.

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  IHC staining of human colon cancer using 29441-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29441-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 29441-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29441-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 29441-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29441-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, MDA-MB-231 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

29441-1-AP targets YTHDC1 in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YTHDC1 fusion protein Ag31459
• Full Name: YTH domain containing 1
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: ~100 kDa
• GenBank Accession Number: BC053863
• Gene Symbol: YTHDC1
• Gene ID (NCBI): 91746
• RRID: AB_2918307
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YTHDC1, containing 1 YTH domain, is one of the m6A-binding proteins that can recognize and bind to the m6A methylation site and plays a specific role in gene expression. YTHDC1 is constitutively enriched in the nucleus. It regulates mRNA splicing by bridging the interactions between the trans- and cis-regulatory elements to bind targeted mRNAs. YTHDC1 mediates the export of m6A modified mRNA from the nucleus to the cytoplasm by interacting with SRSF3, which is an essential factor driving the tumorigenic process in various types of cancers including breast cancer, colon cancer, ovarian cancer, osteosarcoma, and glioblastoma (32368386).

Publications

Species	Application	Title
human	WB	Cancer Cell Int; YTHDC1-mediated VPS25 regulates cell cycle by targeting JAK-STAT signaling in human glioma cells.; Authors - Xiaolong Zhu ; KD Validated
mouse	WB	J Clin Lab Anal; Mettl3 promotes oxLDL-mediated inflammation through activating STAT1 signaling.; Authors - Zhenwei Li
human	WB	Nanoscale Res Lett; RNA m6A Alterations Induced by Biomineralization Nanoparticles: A Proof-of-Concept Study of Epitranscriptomics for Nanotoxicity Evaluation.; Authors - Jinbin Pan
human	WB	Discov Oncol; Systematic analysis of the expression profile and prognostic significance of m6A regulators and PD-L1 in hepatocellular carcinoma; Authors - Fanhua Kong
human	RIP	Cell Stem Cell; Decoding m6A RNA methylome identifies PRMT6-regulated lipid transport promoting AML stem cell maintenance; Authors - Ying Cheng
human	WB,IHC,IP	Exp Mol Med; The YTHDC1/GLUT3/RNF183 axis forms a positive feedback loop that modulates glucose metabolism and bladder cancer progression; Authors - Bin Yan ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zhongwen (Verified Customer) (09-25-2023)

Clear band around 100KD

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

YTHDC1 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:29441-1-AP)