YTHDC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

YTHDC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

35

Cat no : 14392-1-AP

Synonyms

KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1

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Validation Data Gallery

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  WB analysis of HEK-293 using 14392-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14392-1-AP (YTHDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse tissue using 14392-1-AP

  WB result of YTHDC1 (14392-1-AP) antibody with mouse sample.

• 

  WB analysis of MCF-7 using 14392-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14392-1-AP (YTHDC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 14392-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14392-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 14392-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14392-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14392-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14392-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14392-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14392-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, mouse tissue
• Positive IHC detected in: mouse brain tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 28 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 7 publications below

Product Information

14392-1-AP targets YTHDC1 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YTHDC1 fusion protein Ag5882
• Full Name: YTH domain containing 1
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: ~100 kDa
• GenBank Accession Number: BC053863
• Gene Symbol: YTHDC1
• Gene ID (NCBI): 91746
• RRID: AB_2878052
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YTHDC1, also named as KIAA1966 or YT521, is a 727 animo acid protein, which contains 1 YTH domain. YTHDC1 localizes in the nucleus and Interacts with EMD, KHDRBS1/SAM68, KHDRBS3, TRA2B/SFRS10, SC35 and SF2. YTHDC1 as a RNA-binding protein that regulates alternative splice site selection. The calcualted molecular weight of YTHDC1 is 85 kDa, but modified TFEB is about 110 kDa (PMID: 10973987 ).

Publications

Species	Application	Title
mouse	WB	Nature; m6A facilitates hippocampus-dependent learning and memory through YTHDF1.; Authors - Hailing Shi
human	WB	Nat Commun; Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.; Authors - Shujie Chen
human	WB,IF	Nucleic Acids Res; N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication.; Authors - Haojie Hao
human	WB	Cell Death Differ; YTHDC1-mediated augmentation of miR-30d in repressing pancreatic tumorigenesis via attenuation of RUNX1-induced transcriptional activation of Warburg effect.; Authors - Yichao Hou
mouse	RIP	Dev Cell; N6-Adenosine Methylation of Socs1 mRNA Is Required to Sustain the Negative Feedback Control of Macrophage Activation.; Authors - Jie Du
human	WB,IP	Mol Ther Nucleic Acids; METTL14-mediated Lnc-LSG1 m6A modification inhibits clear cell renal cell carcinoma metastasis via regulating ESRP2 ubiquitination.; Authors - Danyang Shen ; KD Validated