XCL1 Polyclonal antibody

XCL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26605-1-AP

Synonyms

Lymphotactin, Small-inducible cytokine C1, SCM-1-alpha, Cytokine SCM-1, C motif chemokine 1

---

Validation Data Gallery

• 

  WB analysis using 26605-1-AP

  Untreated, PMA, Ionomycin and Brefeldin A treated NK-92 cells were subjected to SDS PAGE followed by western blot with 26605-1-AP (Lymphotactin antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 26605-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26605-1-AP (XCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: PMA, Ionomycin and Brefeldin A treated NK-92 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26605-1-AP targets XCL1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XCL1 fusion protein Ag24258
• Full Name: chemokine (C motif) ligand 1
• Calculated Molecular Weight: 114 aa, 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC070309
• Gene Symbol: XCL1
• Gene ID (NCBI): 6375
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XCL1 also known as Lymphotactin, is a member of the chemokine superfamily. Chemokines function in inflammatory and immunological responses, inducing leukocyte migration and activation.