XBP1S-specific Recombinant antibody

XBP1S-specific Uni-rAb^TM Recombinant Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241004C10

Cat no : 83959-6-RR

Synonyms

XBP1, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1, X box binding protein 1, TREB-5

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Validation Data Gallery

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  IHC staining of mouse liver using 83959-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83959-6-RR (XBP1S-specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 83959-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83959-6-RR (XBP1S-specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83959-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83959-6-RR against Human XBP1S-specific were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83959-6-RR is XBP1S-specific Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: XBP1S-specific fusion protein Ag21703
• Full Name: X-box binding protein 1
• Calculated Molecular Weight: 261 aa, 29 kDa
• GenBank Accession Number: BC000938
• Gene Symbol: XBP1
• Gene ID (NCBI): 7494
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XBP1, also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). XBP1 localizes in the nucleus. As a transcription factor, it is essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response. XBP1 has an association with major affective disorder. The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues.