XBP-1U specific Polyclonal antibody

• Featured Product
• KD/KO Validated

XBP-1U specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

46

Cat no : 25997-1-AP

Synonyms

XBP1, X-box-binding protein 1, luminal form, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1, X box binding protein 1

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Validation Data Gallery

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  WB analysis of rat spleen using 25997-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 25997-1-AP (XBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 25997-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 25997-1-AP (XBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 25997-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25997-1-AP (XBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 25997-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 25997-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 25997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 25997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25997-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25997-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25997-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 25997-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 25997-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XBP-1U specific (25997-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: rat spleen tissue, mouse spleen tissue
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 42 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Product Information

25997-1-AP targets XBP-1U specific in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: XBP-1U specific fusion protein Ag21714
• Full Name: X-box binding protein 1
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC000938
• Gene Symbol: XBP1
• Gene ID (NCBI): 7494
• RRID: AB_2880326
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

X-box-binding protein 1 (XBP1), also named as TREB5, is a 261 amino acid protein, which contains one bZIP domain and belongs to the bZIP family. XBP1 localizes in the nucleus and as a transcription factor is essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response. XBP1 has an association with major affective disorder. The molecular weight of protein generated from the spliced XBP1 mRNA is 54 kDa and protein generated from unspliced Xbp1 mRNA is 33 kDa. This antibody 25997-1-AP specially recognizes isoform XBP-1U.

Publications

Species	Application	Title
human	WB	J Extracell Vesicles; A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells.; Authors - Charles P Hinzman
mouse	WB	Hepatology; Farnesoid X receptor signaling activates the hepatic X-box binding protein 1 pathway in vitro and in mice.; Authors - Xiaoying Liu
human	IHC,IF	Sci Adv; Identification of XBP1-u as a novel regulator of the MDM2/p53 axis using an shRNA library.; Authors - Can Huang
mouse	WB	Acta Pharm Sin B; The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.; Authors - Weimin Yu
pig	WB	Autophagy; Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy.; Authors - Sheng-Li Ming
human	WB	Oncogene; Knockdown of TM9SF4 boosts ER stress to trigger cell death of chemoresistant breast cancer cells.; Authors - Yifei Zhu