WNT6 Polyclonal antibody

• Featured Product
• KD/KO Validated

WNT6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 24201-1-AP

Synonyms

Protein Wnt-6, Protein Wnt 6

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Validation Data Gallery

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  WB analysis of SGC-7901 using 24201-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24201-1-AP (WNT6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 24201-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24201-1-AP (WNT6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 24201-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24201-1-AP (WNT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 24201-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24201-1-AP (WNT6 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 24201-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24201-1-AP (WNT6 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: SGC-7901 cells
• Positive IHC detected in: mouse brain tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

24201-1-AP targets WNT6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WNT6 fusion protein Ag20889
• Full Name: wingless-type MMTV integration site family, member 6
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC004329
• Gene Symbol: WNT6
• Gene ID (NCBI): 7475
• RRID: AB_2879454
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WNT6 belongs to the Wnt family. It is ligand for members of the frizzled family of seven transmembrane receptors. WNT6 is likely to signal over only few cell diameters. WNT6 may be together with CAV1 to promote chemoresistance of gastric cancer cells to DNA-damaging anthracycline drugs through the activation of the canonical Wnt receptor signaling pathway. And WNT6 is mainly expressed in brain, endometrium epithelium, gastric cancer cell lines and gastric cancer tissues (PMID: 22370641).

Publications

Species	Application	Title
human,chicken	IHC,IF	Development; Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.; Authors - Jianqiong Lin
human	WB	Front Oncol; YTHDF1 Regulates Tumorigenicity and Cancer Stem Cell-Like Activity in Human Colorectal Carcinoma.; Authors - Yang Bai
human	WB	Am J Physiol Cell Physiol; Secreted Wnt6 mediates diabetes-associated centrosome amplification via its receptor FZD4.; Authors - Qin Ju He
human	WB,IHC,IF	Gene; WNT6 participates in the occurrence and development of ovarian cancer by upregulating/activating the typical Wnt pathway and Notch1 signaling pathway; Authors - Haijuan Bao ; KD Validated
human	WB,IHC	Oncol Lett; Correlation study of DNA methylation of WNT6 gene with osteosarcoma in children.; Authors - Li Li
human	WB	Clin Transl Med; LncRNA NEAT1 suppresses cellular senescence in hepatocellular carcinoma via KIF11-dependent repression of CDKN2A; Authors - Danlei Chen