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WNT6 Polyclonal antibody
WNT6 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 24201-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | SGC-7901 cells |
Positive IHC detected in | mouse brain tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 5 publications below |
IHC | See 4 publications below |
IF | See 2 publications below |
Product Information
24201-1-AP targets WNT6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, chicken |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | WNT6 fusion protein Ag20889 |
Full Name | wingless-type MMTV integration site family, member 6 |
Calculated Molecular Weight | 39 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | BC004329 |
Gene Symbol | WNT6 |
Gene ID (NCBI) | 7475 |
RRID | AB_2879454 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
WNT6 belongs to the Wnt family. It is ligand for members of the frizzled family of seven transmembrane receptors. WNT6 is likely to signal over only few cell diameters. WNT6 may be together with CAV1 to promote chemoresistance of gastric cancer cells to DNA-damaging anthracycline drugs through the activation of the canonical Wnt receptor signaling pathway. And WNT6 is mainly expressed in brain, endometrium epithelium, gastric cancer cell lines and gastric cancer tissues (PMID: 22370641).
Protocols
Product Specific Protocols | |
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WB protocol for WNT6 antibody 24201-1-AP | Download protocol |
IHC protocol for WNT6 antibody 24201-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Development Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch. | ||
Front Oncol YTHDF1 Regulates Tumorigenicity and Cancer Stem Cell-Like Activity in Human Colorectal Carcinoma. | ||
Am J Physiol Cell Physiol Secreted Wnt6 mediates diabetes-associated centrosome amplification via its receptor FZD4. | ||
Gene WNT6 participates in the occurrence and development of ovarian cancer by upregulating/activating the typical Wnt pathway and Notch1 signaling pathway
| ||
Oncol Lett Correlation study of DNA methylation of WNT6 gene with osteosarcoma in children. | ||
Clin Transl Med LncRNA NEAT1 suppresses cellular senescence in hepatocellular carcinoma via KIF11-dependent repression of CDKN2A |