WNT5A/B Polyclonal antibody

WNT5A/B Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 29793-1-AP

Synonyms

hWNT5A, Protein Wnt 5a, WNT5A, WNT5A B

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Validation Data Gallery

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  WB analysis using 29793-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29793-1-AP (WNT5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 29793-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WNT5A antibody (29793-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, SKOV-3 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

29793-1-AP targets WNT5A/B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WNT5A/B fusion protein Ag31507
• Full Name: wingless-type MMTV integration site family, member 5A
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC064694
• Gene Symbol: WNT5A
• Gene ID (NCBI): 7474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathway. Wnt5A is a member of the Wnt family of proteins, which are 38-45 kDa secreted cysteine-rich proteins with hydrophobic signal peptides.

Publications

Species	Application	Title
rat	WB	J Biol Chem; Exosomal miR-23a-3p derived from human umbilical cord mesenchymal stem cells promotes remyelination in central nervous system demyelinating diseases by targeting Tbr1/Wnt pathway; Authors - Danqing Qin
human	WB,IHC	J Orthop Res; Increased Wnt5a/ROR2 signaling is associated with chondrogenesis in meniscal degeneration; Authors - Yusuke Inoue
rat	WB	Biochim Biophys Acta Mol Cell Res; Wnt/β-catenin signaling inhibits oxidative stress-induced ferroptosis to improve interstitial cystitis/bladder pain syndrome by reducing NF-κB; Authors - Weilin Fang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (12-22-2023)

Worked well for WB at 1:2000 in ADSCs

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: ADSCs