WNT3A Polyclonal antibody

WNT3A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26744-1-AP

Synonyms

Protein Wnt 3a, WNT3A



Tested Applications

Positive WB detected infetal human brain tissue, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 25 publications below

Product Information

26744-1-AP targets WNT3A in WB, ELISA applications and shows reactivity with human, Mouse samples.

Tested Reactivity human, Mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WNT3A fusion protein Ag25051
Full Name wingless-type MMTV integration site family, member 3A
Calculated Molecular Weight 352 aa, 39 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC103921
Gene Symbol WNT3A
Gene ID (NCBI) 89780
RRIDAB_2918108
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WNT3A belongs to wingless-type MMTV integration site family, and abnormal Wnt signalling is often associated with severe human diseases, including cancer, osteoporosis and other degenerative disorders (PMID: 20716463). Wnt3a, was shown to promote trophectoderm formation in embryonic stem cells by inducing Cdx2, one of the critical transcription factors of early trophoblast development, in an LEF-1-dependent manner. Wnt3a may also activate other β-catenin-independent cascades such as ERK or PI3K/AKT signalling, again emphasizing the complexity of the Wnt signalling network.

Protocols

Product Specific Protocols
WB protocol for WNT3A antibody 26744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Aging (Albany NY)

Enriched environment remedies cognitive dysfunctions and synaptic plasticity through NMDAR-Ca2+-Activin A circuit in chronic cerebral hypoperfusion rats.

Authors - Xin Zhang
ratWB

Arch Toxicol

Developmental exposure to nonylphenol induced rat axonal injury in vivo and in vitro.

Authors - Siyao Li
humanWB

Apoptosis

SKI knockdown suppresses apoptosis and extracellular matrix degradation of nucleus pulposus cells via inhibition of the Wnt/β-catenin pathway and ameliorates disc degeneration.

Authors - Zuo-Long Wu
humanWB

Biomed Pharmacother

BAIAP2L2 promotes the progression of gastric cancer via AKT/mTOR and Wnt3a/β-catenin signaling pathways.

Authors - Jianing Liu
humanWB

J Cancer

Demethylzelasteral inhibits proliferation and EMT via repressing Wnt/β-catenin signaling in esophageal squamous cell carcinoma.

Authors - Jiarui Yu
humanWB

Toxicol Lett

Bavachin induces liver injury and cell apoptosis by targeting Wnt/β-catenin/DRP1 signaling pathway mediated mitochondrial dysfunction

Authors - Ying Yang