WIPI1 Polyclonal antibody

• Featured Product
• KD/KO Validated

WIPI1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25204-1-AP

Synonyms

WIPI-1, WIPI 49 kDa, WIPI 1, WD repeat domain phosphoinositide-interacting protein 1, Atg18 protein homolog

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Validation Data Gallery

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  WB analysis of SW 1990 using 25204-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 25204-1-AP (WIPI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 25204-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25204-1-AP (WIPI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25204-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25204-1-AP (WIPI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SW 1990 using 25204-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 25204-1-AP (WIPI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 25204-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25204-1-AP (WIPI1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 25204-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25204-1-AP (WIPI1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 25204-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25204-1-AP (WIPI1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 25204-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25204-1-AP (WIPI1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SW 1990 cells, HEK-293 cells, NIH/3T3 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Product Information

25204-1-AP targets WIPI1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WIPI1 fusion protein Ag18461
• Full Name: WD repeat domain, phosphoinositide interacting 1
• Calculated Molecular Weight: 446 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC039867
• Gene Symbol: WIPI1
• Gene ID (NCBI): 55062
• RRID: AB_2879958
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WIPI1, or WD repeat domain, phosphoinositide-interacting protein 1, is a member of the WD40 repeat family of proteins, which are key components of many essential biological functions. WIPI1 is involved in the regulation of autophagy, a cellular process critical for maintaining cellular integrity and recycling intracellular proteins, lipids, and organelles. WIPI1 is characterized by its 7-bladed β-propeller structure and contains a conserved motif for interaction with phospholipids, specifically binding to phosphoinositides. This interaction is crucial for its function in autophagy, where it acts as an effector of phosphatidylinositol 3-phosphate (PtdIns3P). WIPI1 and WIPI2 are among the first proteins to be recruited during autophagy, with WIPI2 interacting with components of the ULK1/2 complex and PtdIns3P, tethering the phagophore to the endoplasmic reticulum (ER). WIPI1 supports the function of WIPI2 in recruiting the ATG16L complex, which is essential for LC3 lipidation and autophagosome formation.

Publications

Species	Application	Title
human	WB,IP	Oral Oncol; WIPI-1 inhibits metastasis and tumour growth via the WIPI-1-TRIM21 axis and MYC regulation in nasopharyngeal carcinoma.; Authors - Yin Zhao ; KD Validated