WFS1 Monoclonal antibody

WFS1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4C2B5

Cat no : 67291-1-Ig

Synonyms

FLJ51211, WFRS, WFS, WFS1, Wolfram syndrome 1 (wolframin), WOLFRAMIN

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Validation Data Gallery

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  WB analysis using 67291-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A172 using 67291-1-Ig

  A172 cells were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67291-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67291-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells, A172 cells, HT-29 cells, HEK-293 cells, C6 cells, U-251 cells, PC-12 cells, Neuro-2a cells, SW480 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67291-1-Ig targets WFS1 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: WFS1 fusion protein Ag25735
• Full Name: Wolfram syndrome 1 (wolframin)
• Calculated Molecular Weight: 890 aa, 100 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC030130
• Gene Symbol: WFS1
• Gene ID (NCBI): 7466
• RRID: AB_2882556
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.