HE4 Polyclonal antibody

HE4 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14406-1-AP

Synonyms

WFDC2, WAP5, WAP four-disulfide core domain protein 2, WAP four disulfide core domain protein 2, Major epididymis-specific protein E4



Tested Applications

Positive IHC detected inhuman ovary tumor tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14406-1-AP targets HE4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HE4 fusion protein Ag5684
Full Name WAP four-disulfide core domain 2
Calculated Molecular Weight 13 kDa
GenBank Accession NumberBC046106
Gene Symbol HE4
Gene ID (NCBI) 10406
RRIDAB_2878055
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.

Protocols

Product Specific Protocols
IHC protocol for HE4 antibody 14406-1-APDownload protocol
IF protocol for HE4 antibody 14406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Biosens Bioelectron

An ultrasensitive ratiometric electrochemiluminescence immunosensor combining photothermal amplification for ovarian cancer marker detection.

Authors - Dandan Fang
mouseWB

Oncotarget

Bcl-3 is a novel biomarker of renal fibrosis in chronic kidney disease.

Authors - Ran Chen
mouseWB

Food Funct

EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.

Authors - Jian Fang
human

RSC Adv

Machine learning algorithms enhance the specificity of cancer biomarker detection using SERS-based immunoassays in microfluidic chips

Authors - Nariman Banaei
humanIHC

Iran J Pathol

HE4, A New Potential Tumor Marker for Early Diagnosis and Predicting of Breast Cancer Progression.

Authors - Nazanin Mirmohseni Namini
humanIHC

Cell Rep

YAP targetome reveals activation of SPEM in gastric pre-neoplastic progression and regeneration

Authors - Adrian K H Loe