WASP Recombinant antibody, PBS Only (Detector)

WASP Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242074E4

Cat no : 84714-2-PBS

Synonyms

Wiskott-Aldrich syndrome protein, WAS, Actin nucleation-promoting factor WAS, 242074E4

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Validation Data Gallery

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  WB analysis using 84714-2-RR (same clone as 84714-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84714-2-RR (WASP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84714-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01520-1

  Cytometric bead array standard curve of MP01520-1, WASP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84714-1-PBS. Detection antibody: 84714-2-PBS. Standard: Ag1444. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84714-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84714-2-RR against Human WASP were performed. The affinity constant is 1.88 nM.

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Product Information

84714-2-PBS targets WASP as part of a matched antibody pair:

MP01520-1: 84714-1-PBS capture and 84714-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: WASP fusion protein Ag1444
• Full Name: Wiskott-Aldrich syndrome (eczema-thrombocytopenia)
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC012738
• Gene Symbol: WASP
• Gene ID (NCBI): 7454
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Wiskott-Aldrich Syndrome protein (WASP) regulates actin cytoskeleton in hematopoietic cells and defects in WASP cause Wiskott-Aldrich Syndrome (WAS), an X-linked immunodeficiency and autoimmunity disorder of childhood.