WASP Polyclonal antibody

• Featured Product
• KD/KO Validated

WASP Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (2)

Cat no : 10987-1-AP

Synonyms

Wiskott-Aldrich syndrome protein, WAS, Actin nucleation-promoting factor WAS

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Validation Data Gallery

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  WB analysis using 10987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10987-1-AP (WASP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10987-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10987-1-AP (WASP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 10987-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10987-1-AP (WASP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10987-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10987-1-AP (WASP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 10987-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10987-1-AP (WASP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 10987-1-AP

  IP result of anti-WASP (IP:10987-1-AP, 4ug; Detection:10987-1-AP 1:5000) with Raji cells lysate 1600 ug.

• 

  IP experiment of rat spleen using 10987-1-AP

  IP result of anti-WASP (IP:10987-1-AP, 4ug; Detection:10987-1-AP 1:500) with rat spleen tissue lysate 4000 ug.

• 

  IHC staining of human spleen using 10987-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10987-1-AP (WASP antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 10987-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10987-1-AP (WASP antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10987-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10987-1-AP (WASP antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10987-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10987-1-AP (WASP antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 10987-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10987-1-AP (WASP antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 10987-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10987-1-AP (WASP antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 10987-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10987-1-AP (WASP antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HL-60 cells, K-562 cells, mouse spleen tissue, Jurkat cells, Raji cells
• Positive IP detected in: Raji cells, rat spleen tissue
• Positive IHC detected in: human tonsillitis tissue, human lymphoma tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

10987-1-AP targets WASP in WB, IHC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WASP fusion protein Ag1444
• Full Name: Wiskott-Aldrich syndrome (eczema-thrombocytopenia)
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 55-62 kDa
• GenBank Accession Number: BC012738
• Gene Symbol: WASP
• Gene ID (NCBI): 7454
• RRID: AB_2304546
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Wiskott-Aldrich Syndrome protein (WASP) regulates actin cytoskeleton in hematopoietic cells and defects in WASP cause Wiskott-Aldrich Syndrome (WAS), an X-linked immunodeficiency and autoimmunity disorder of childhood.

Publications

Species	Application	Title
mouse	WB	J Allergy Clin Immunol; Dedicator of cytokinesis protein 2 couples with lymphoid enhancer-binding factor 1 to regulate expression of CD21 and B-cell differentiation.; Authors - Yukai Jing
human		Nat Commun; WASp modulates RPA function on single-stranded DNA in response to replication stress and DNA damage.; Authors - Seong-Su Han
human	WB	J Exp Clin Cancer Res; miR-182 suppresses invadopodia formation and metastasis in non-small cell lung cancer by targeting cortactin gene.; Authors - Yongwen Li ; KD Validated
human	WB	J Exp Clin Cancer Res; CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling.; Authors - Dong-Fang Meng
human	WB,RIP,IP	Mol Cancer Res; LINC00869 Promotes Hepatocellular Carcinoma Metastasis via Protrusion Formation; Authors - Xiaowen Shao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Seong-Su (Verified Customer) (09-17-2020)

I checked nine WASp Abs from 4 different companies for ELISA and EMSA super-shift. Among them this proteintech WASp Ab showed wonderful result for ELISA as well as was the only Ab that showed shifted bands of WASp binding complex.My colleague used this Ab for western and got a beautiful result.

• Applications: Western Blot, Other
• Primary Antibody Dilution: ELISA: 1/100 dilution, EMSA super-shift: no dilution
• Cell Tissue Type: immortalized T or B cells / B lymphoma cell lines / Jurkat

FH

Nisreen (Verified Customer) (11-07-2017)

The antibody are clean and nice.

• Applications: Western Blot