ChromoTek Vimentin-Label Atto488

anti-vimentin VHH/ Nanobody conjugated to the fluorophore ATTO488 for IF, & microscopy

Specificity

Vimentin

Applications

IF, ICC

Conjugate

ATTO 488

Host

Alpaca

Publications

3

Cat no : vba488

Synonyms

Vimentin-Label, VIM, Vimentin

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Validation Data Gallery

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  MDCK cells were immunostained with Vimentin-Booster (in green), cell nuclei were counterstained with DAPI (in blue).

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Product Information

anti-vimentin VHH/ Nanobody conjugated to the fluorophore ATTO488 for IF, & microscopy

• Description: Vimentin-Label specifically binds to the Vimentin intermediate filament protein. Due to its small size, the Vimentin-Labelenables higher image quality in epifluorescence, confocal, and super-resolution microscopy:

  • Specific probe for direct immunostaining of Vimentin filaments

  • Higher image resolution

  • Less than 4 nm epitope-label displacement minimizes linkage error

  • Superior accessibility and labeling of epitopes in crowded cellular/organelle environments

  • Improved target binding due to an avidity effect from the bivalent form of Vimentin-Label

• Applications: Monoclonal
• Host: Alpaca
• Specificity/Target: Vimentin-Label specifically binds to the vimentin intermediate filament protein. Validated on canine MDCK cells, rodent BHK cells and on human A549 cells upon stimulation with TGFβ.
• Conjugate: ATTO 488
• Physical State: Liquid
• Suggested Dilution: IF/ICC: 1:200 – 1:400
• Type: Nanobody
• Class: Recombinant
• RRID: AB_2631392
• Storage Buffer: 1x PBS, 0.09% sodium azide.
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Do not freeze. Protect from light.
• Note: This product is for research use only, not for diagnostic or therapeutic use

Publications

Application	Title
IF	Nat Neurosci; Panoptic imaging of transparent mice reveals whole-body neuronal projections and skull-meninges connections.; Authors - Ruiyao Cai
IF	Light Sci Appl; Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy.; Authors - Jing Liu
IF	bioRxiv; Genetic removal of Nlrp3 protects against sporadic and R345W Efemp1-induced basal laminar deposit formation; Authors - Antonio J Ortega