ChromoTek Vimentin-Chromobody® plasmid (TagGFP)

DNA plasmid encoding for anti-Vimentin VHH (anti-Vimentin Nanobody) fused to TagGFP2.

Specificity

Vimentin

Applications

IF, Live cell imaging

Vector type

Mammalian expression vector

Reporter

TagGFP2

Publications

2

Cat no : vcg

Synonyms

Chromobody, Vimentin Chromobody

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Validation Data Gallery

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  Visualize vimentin cytoskeleton with the Vimentin-Chromobody in real time in your cell line of interest

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  Vimentin-Chromobody visualizes Vimentin filaments in live cells

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Product Information

DNA plasmid encoding for anti-Vimentin VHH (anti-Vimentin Nanobody) fused to TagGFP2.

• Description: The Vimentin-Chromobody visualizes endogenous Vimentin in real time.

  • Easily transfect your cells with the Vimentin-Chromobody plasmid

  • Use Vimentin-Chromobody as a marker of vimentin cytoskeleton

  • Monitor dynamics of vimentin intermediate filaments in live cells

  • Vimentin-Chromobody does not affect cell viability

  • The binding of Vimentin-Chromobody does not influence cell viability or cell motility and thus offers you the unique possibility to non-invasively monitor vimentin cytoskeleton in your cells.

• Applications: Trace endogenous vimentin in real-time; Live cell imaging; Monitor epithelial-mesenchymal transition in live cells
• Specificity: Vimentin intermediate filament protein, tested in human, hamster & dog cells
• Reporter: TagGFP2
• Vector type: Mammalian expression vector
• Promoter: Constitutive CMV IE
• Codon usage: Mammalian
• Selection: Kan/Neo
• Sequence: With the Vimentin-Chromobody plasmid you receive the sequence information of the alpaca antibody to Vimentin fused to TagGFP2 or TagRFP, as well as the full vector sequence.
• Transfection: Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
• Microscopy techniques: Wide-field epifluorescence microscopy; confocal microscopy
• Storage Condition: Shipped at ambient temperature. Store at -20 °C

Publications

Application	Title
IF	Cancer Res; Visualizing Epithelial-Mesenchymal Transition Using the Chromobody Technology.; Authors - Julia Maier
	Methods Cell Biol; Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos.; Authors - Catherine S Schrankel