Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI Method)

PI:Ex/Em (with DNA)

535/617 nm

Calcein AM：Ex/Em

494/517 nm

Application

FC

Publications

23

Cat no : PF00007

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Validation Data Gallery

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  Q2-UL：Cell Group with Calcein AM -, EthD-I + (dead cells)；
  Q2-LL：Cell Group with Calcein AM-, PI - (cell debris)；
  Q2-LR：Cell Group with Calcein AM +, E PI - (live cells).
  

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Product Information

Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI method) provides dual fluorescence staining for the detection of living and dead cells. The two probes in the kit eflect cell viability by measuring intracellular esterase activity and plasma membrane integrity. This kit can be used for fluorescence microscopes, flow cytometers, microplate readers and other fluorescence detection systems. 

This kit can be used for most eukaryotic mammalian samples, but not to fungi and yeast.

Components	150T	300T
A. Calcein AM (4 mM in anhydrous DMSO)	50 uL	100 uL
B. Propidium (PI) (1.5 mM in H2O)	250 uL	500 uL

Note: The number of times(T) of this kit is specified according to the usage of 0.5mL working solution for one sample of flow cytometry.

Storage

Store at -20°C. Avoid exposure to light. Stable for 24 months after shipment. 

Protocol

Viability/Cytotoxicity cell staining kit (Calcein AM, PI) Protocol

Cautions

1. Fluorescent dyes all have quenching problems.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

Parameters

CalceinAM：Ex/Em=494/517nm

PI: Ex/Em=535/617nm (with DNA)

Cited in Article as

PF00007, Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, PI Method), Proteintech, IL, USA

Publications

Application	Title
	ACS Nano; Engineered Extracellular Vesicle-Delivered CRISPR/Cas9 for Radiotherapy Sensitization of Glioblastoma; Authors - Xiao Liu
	Adv Sci (Weinh); Regulating Type H Vessel Formation and Bone Metabolism via Bone-Targeting Oral Micro/Nano-Hydrogel Microspheres to Prevent Bone Loss; Authors - Junjie Li
	Adv Healthc Mater; Long-lived Photoacid-doped Conducting Composites Induce Photocurrent for Efficient Wound Healing; Authors - Haiyan Yin
	FASEB J; HDM induce airway epithelial cell ferroptosis and promote inflammation by activating ferritinophagy in asthma.; Authors - Zhaojin Zeng
	J Cardiovasc Transl Res; CD137 Signal Mediates Cardiac Ischemia-Reperfusion Injury by Regulating the Necrosis of Cardiomyocytes.; Authors - Yao Xu
	Drug Des Devel Ther; Curcumin- and Cyclopamine-Loaded Liposomes to Enhance Therapeutic Efficacy Against Hepatic Fibrosis.; Authors - Ting Zhang