VEGFA Recombinant antibody

VEGFA Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G5

Cat no : 82864-16-RR

Synonyms

Vpf, Vegf a, Vegf, vascular endothelial growth factor A, Vascular Endothelial Growth Factor

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Validation Data Gallery

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  WB analysis using 82864-16-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82864-16-RR (Vegfa antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82864-16-RR

  Biolayer interferometry (BLl) kinetic assays of 82864-16-RR against Mouse VEGFA were performed. The affinity constant is 0.505 nM.

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Tested Applications

• Positive WB detected in: MCF-7 cells, T-47D cells, MDA-MB-231 cells, HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82864-16-RR targets VEGFA in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: vascular endothelial growth factor A
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: NM_001287056
• Gene Symbol: Vegfa
• Gene ID (NCBI): 22339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).