Vegfa Polyclonal antibody

Vegfa Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 26157-1-AP

Synonyms

Vegf, Vegf a, Vegf120, Vegf164, Vegf188, Vegfa, Vpf

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Validation Data Gallery

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  WB analysis of mouse brain using 26157-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26157-1-AP (Vegfa Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26157-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 26157-1-AP (Vegfa antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse lung using 26157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26157-1-AP (Vegfa antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse lung using 26157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26157-1-AP (Vegfa antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 1 publications below

Product Information

26157-1-AP targets Vegfa in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Vegfa fusion protein Ag24084
• Full Name: vascular endothelial growth factor A
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: NM_001287056
• Gene Symbol: Vegfa
• Gene ID (NCBI): 22339
• RRID: AB_2880408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vegfa is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis.It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. It may play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.

Publications

Species	Application	Title
rat	WB	Respir Res; The role of RNA m6A methylation in the regulation of postnatal hypoxia-induced pulmonary hypertension.; Authors - Shanshan Xu
human	WB	Aging (Albany NY); Transplanting Rac1-silenced bone marrow mesenchymal stem cells promote neurological function recovery in TBI mice.; Authors - Dongdong Huang
mouse	WB	Cell Biol Int; Involvement of HIF1 stabilization and VEGF signaling modulated by Grx-1 in murine model of bronchopulmonary dysplasia; Authors - Wenli Han
mouse	WB	Biochem Biophys Res Commun; Ablation of glutaredoxin 1 promotes pulmonary angiogenesis and alveolar formation in hyperoxia-injured lungs by modifying HIF-1α stability and inhibiting the NF-κB pathway.; Authors - Xuwei Liu
human	WB	J Surg Res; Hypoxia-Inducible Factor 1α Stability Modified by Glutaredoxin-1 in Necrotizing Enterocolitis; Authors - Yunfei Zhang
human	WB,IHC	Cell Death Dis; LncRNA ZNFTR functions as an inhibitor in pancreatic cancer by modulating ATF3/ZNF24/VEGFA pathway.; Authors - Wei Li