VWF Monoclonal antibody, PBS Only

VWF Monoclonal Antibody for IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F9F3

Cat no : 66682-1-PBS

Synonyms

F8VWF, von Willebrand antigen II, von Willebrand factor, VWD, VWF

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Validation Data Gallery

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  IHC staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66682-1-Ig (VWF antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66682-1-Ig (VWF antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using VWF antibody (66682-1-Ig, Clone: 3F9F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using VWF antibody (66682-1-Ig, Clone: 3F9F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66682-1-PBS targets VWF in IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: VWF fusion protein Ag25578
• Full Name: von Willebrand factor
• Gene Symbol: VWF
• Gene ID (NCBI): 7450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Von Willebrand factor (VWF) is a large multimeric glycoprotein found in blood plasma involved in hemostasis following vascular injury. Due to the multimeric nature of VWF, it can range in size from 500 to 20,000 kDa due to the differences in the number of subunits comprising the protein. Each subunit is approximately 250 kDa (PMID: 9759493). The biosynthesis of VWF in vivo is limited to endothelial cells (PMID: 4209883) and megakaryocytes (PMID: 2413071). VWF synthesized in endothelial cells is either released directly into the plasma via 27186a secretory pathway, or tubulized and stored in organelles unique to this cell type called Weibel-Palade bodies (PMID: 16459301). Whereas VWF synthesized in megakaryocytes is stored in the alpha granules of platelets (PMID: 2046403). The primary function of VWF is as an adhesive plasma glycoprotein, particularly factor VIII; an essential blood-clotting protein (PMID: 6982084). VWF is also important in platelet adhesion to wound sites by binding specifically to type I and type III collagen (PMID: 11098050), with larger VWF multimers being most effective (PMID: 24448155).