VTA1 Polyclonal antibody
VTA1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 15786-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, A549 cells, HepG2 cells, human urine sample, K-562 cells, rat brain tissue, HSC-T6 cells, SMMC-7721 cells, mouse brain tissue, fetal human brain tissue |
Positive IP detected in | HEK-293 cells |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
15786-1-AP targets VTA1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | VTA1 fusion protein Ag8485 |
Full Name | Vps20-associated 1 homolog (S. cerevisiae) |
Calculated Molecular Weight | 307 aa, 34 kDa |
Observed Molecular Weight | 34 kDa |
GenBank Accession Number | BC006989 |
Gene Symbol | VTA1 |
Gene ID (NCBI) | 51534 |
RRID | AB_2288524 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for VTA1 antibody 15786-1-AP | Download protocol |
IF protocol for VTA1 antibody 15786-1-AP | Download protocol |
IP protocol for VTA1 antibody 15786-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cell Proteomics Proteomic analyses uncover a new function and mode of action for mDia2. |