VPS54 Monoclonal antibody, PBS Only

VPS54 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D12D1

Cat no : 67404-1-PBS

Synonyms

HCC8, hVps54L, SLP 8p, Tumor antigen HOM HCC 8, Tumor antigen SLP 8p, VPS54, VPS54L

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 67404-1-Ig (same clone as 67404-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67404-1-Ig (VPS54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67404-1-Ig (same clone as 67404-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67404-1-Ig (VPS54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67404-1-Ig (same clone as 67404-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67404-1-Ig (VPS54 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67404-1-Ig (same clone as 67404-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67404-1-Ig (VPS54 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

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Product Information

67404-1-PBS targets VPS54 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

• Tested Reactivity: Human, Pig, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: VPS54 fusion protein Ag4123
• Full Name: vacuolar protein sorting 54 homolog (S. cerevisiae)
• Calculated Molecular Weight: 977 aa, 111 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC030275
• Gene Symbol: VPS54
• Gene ID (NCBI): 51542
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

VPS54 is a part of the Golgi-associated retrograde protein (GARP) complex protein required for tethering and fusion of endosome-derived transport vesicles to the trans-Golgi network. It may particpate in retrograde transport from early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.